Museo dell'Opera del Duomo is housed in a 13th century building in Piazza dei Miracoli, just a few metres from the Leaning Tower. The museum first opened in 1986 and was conceived as a location to contain the artefacts that had been removed from the Duomo and the Baptistry, while ensuring their protection and greater public use and accessibility.

The dilemma arose, in the wake of the July 1944 Camposanto monumentale roof fire, of how to best conserve the frescoes of the Camposanto and the relative sinopie (preliminary drawings), which had been revealed in 1974 with the first "tearing" of the overlying paintings.

Located in the medieval monastery of the same name near the river Arno, the National Museum of San Matteo boasts a rich series of works by leading Pisan and Tuscan masters of the 12^th-17^th centuries and a flourishing collection of archaeological and ceramic artifacts. The quantity and value of the exhibits make the San Matteo one of the most important European museums in the field of medieval art.

12^th- and 13^th-century painting is represented by the Croci dipinte (painted crosses), all derived from Pisa’s oldest churches, with works by Berlinghiero Berlinghieri, Giunta Pisano and the Master of San Martino.

The National Museum of the Palazzo Reale is housed in the 16th-century palazzo of the same name, designed by Buontalenti and for centuries home to the reigning families of the Medici, Lorena and Savoia. The palace was renovated in 2001, when spaces and connections with the adjoining buildings were restored.

La Domus Mazziniana, istituita nel 1952, raccoglie l'eredità spirituale e materiale della casa dei Nathan-Rosselli, dove Mazzini fu ospitato fino alla sua morte.

L’edificio fu distrutto nel bombardamento del 31 agosto 1943 e ricostruito nel dopoguerra.

Vi hanno sede il Centro Studi Mazziniani, con un’importante biblioteca sulla storia del Risorgimento, e il museo, che conserva cimeli, medaglie, bandiere, scritti autografi, libri, documenti ed oggetti personali appartenuti a Mazzini.

The Museum of Graphic Arts, set up by the Municipality and University of Pisa, is housed in the Palazzo Lanfranchi, one of the most fascinating buildings of the Lungarni (riverfront), formerly a venue for temporary exhibitions.

The museum preserves the Prints and Drawings Collection of the Department of History of the Arts of the University of Pisa, a collection originating in 1957 through an initiative by Carlo Ludovico Ragghianti which has made Pisa a leading centre of knowledge and study of contemporary art.

Il Museo degli Strumenti per il Calcolo, gestito dalla Fondazione Galileo Galilei,  riproduce un avvincente viaggio nella storia della scienza e rappresenta una realtà unica nella città di Pisa, a pochi passi dalla celeberrima Piazza dei Miracoli.

L’area museale è collocata in uno splendido parco di dodicimila metri quadrati, un’oasi nel cuore di Pisa, dove già nel corso degli anni migliaia di visitatori hanno potuto apprezzare una selezione di grande interesse che si distingue anche nel panorama nazionale non solo per la ricchezza delle collezioni ma anche perché comprende, tra i pezzi più rilevanti, esemplari unici e rappresentativi nella storia del calcolo e dell'informatica.

The Botanic Garden of Pisa university was created in 1543 through the work of the physician and botanist Luca Ghini. It is the oldest university botanic garden in Europe.

Originally situated in the area of the Medici Arsenals, in 1563 it was transferred to the north-west portion of Pisa's historic centre; in 1591 it was moved to its current location. In the 1800s the 16th-century layout was dismantled in favor of a more 'scientific' arrangement of the flower-beds.

The Garden premises enclose the Botanic Institute and the Botanic Museum.

Pisa's Botanic Museum is housed in a small 18th-century building inside the Botanic Garden.

The facade is richly decorated with embedded shells and coloured stones and is quite striking as seen from the Garden.

The museum is a compilation of collections among which particular importance is ascribed to the herbals, collections of pressed and dried plants fixed on sheets of paper and accompanied by information about the plant itself as well as details of where and when it was found.